16S rRNA和recA、groEL基因分类鉴定乳酸乳球菌乳酸亚种和乳脂亚种的比较

Compared on classification and identification of Lactococcus lactis subsp. lactis and cremoris based on 16S rRNA gene and recA, groEL gene

摘要:

【目的】比较16S rRNA和recA、groEL基因部分序列用于乳酸乳球菌乳酸亚种和乳脂亚种分类鉴定的效果。【方法】对已鉴定的8株分离自传统发酵乳的乳酸乳球菌, 选取recA和groEL基因片段, 通过PCR扩增、测序, 将测序得到的序列比对后构建系统发育树, 并与16S rRNA基因序列分析技术进行比较。【结果】比较分析不同菌株16S rRNA和recA、groEL基因的亲缘关系, recA、groEL基因可以准确地完成乳酸乳球菌乳酸亚种和乳脂亚种的区分和鉴定。【结论】recA和groEL基因序列分析可以实现乳酸乳球菌乳酸亚种和乳脂亚种的区分, 因其具有快速、准确、稳定的特点, 可适合于乳酸乳球菌乳酸亚种和乳脂亚种间的快速分类鉴定。

Abstract:

[Objective] Comparing the effects of 16S rRNA gene with recA and groEL gene used in the classification and identification of Lactococcus lactis subsp. lactis and cremoris. [Methods] Eight L. lactis isolated from the traditional fermented milk have been classified by 16S rRNA gene, the recA and groEL genes of these strains were amplified by polymerase chain reaction (PCR), and the PCR products were sequenced, the phylogenetic trees were constructed including 16S rRNA gene and above-mentioned two housekeeping genes. [Results] Comparative analysis of genetic relationship of 16S rRNA gene and the recA, groEL genes, the recA and groEL genes possesses the superiority in classification and identification of L. lactis subsp. lactis and cremoris. [Conclusion] Distinction of L. lactis subsp. lactis and cremoris can be achieved by recA and groEL genes sequence analysis. Due to the fast, accurate and stable characteristics, which were more appropriate for differentiation of L. lactis subsp. lactis and cremoris at the levels of subspecies.