科微学术
微生物学通报
2025年5月12日 周一
国家自然科学基金项目(No. 31070103); 广东省自然科学基金重点项目(No. 10251007002000001); 广东省科技攻关项目(No. 2009B030802013)
LONG Mei-Rong
1. Xinjiang Production & Struction Corps Key Laboratory of Protection & Utilization of Biological Resources in Tarim Basin, College of Life Science, Tarim University, Alar, Xinjiang 843300, China 2. Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Col-lection and Application, Guangdong Open Laboratory of Applied Microbiology, State Key Laboratory of Ap-plied Microbiology (Ministry-Guangdong Province Jointly Breeding Base), South China, Guangzhou, Guangdong 510070, ChinaXIE Xiao-Lin
2. Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Col-lection and Application, Guangdong Open Laboratory of Applied Microbiology, State Key Laboratory of Ap-plied Microbiology (Ministry-Guangdong Province Jointly Breeding Base), South China, Guangzhou, Guangdong 510070, ChinaFENG Guang-da
2. Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Col-lection and Application, Guangdong Open Laboratory of Applied Microbiology, State Key Laboratory of Ap-plied Microbiology (Ministry-Guangdong Province Jointly Breeding Base), South China, Guangzhou, Guangdong 510070, ChinaZHU Hong-hui
2. Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Col-lection and Application, Guangdong Open Laboratory of Applied Microbiology, State Key Laboratory of Ap-plied Microbiology (Ministry-Guangdong Province Jointly Breeding Base), South China, Guangzhou, Guangdong 510070, China【目的】旨在从重金属污染地分离出耐重金属镉真菌, 获得耐受重金属镉污染的高效菌株, 为重金属污染微生物修复提供菌种资源。【方法】利用稀释平板涂布法, 采用4种培养基对粤东北梅州市梅县铅锌尾矿废弃地9个样品进行分离, 并结合形态学和ITS rDNA基因序列分析, 鉴定分离到的耐镉真菌, 最后对分离到的耐镉真菌进行最小抑制浓度(MIC)检测。【结果】从粤东北梅州市梅县铅锌尾矿废弃地分离出72株丝状真菌, 经形态学和分子技术鉴定, 它们主要属于曲霉属(Aspergillus)、青霉属(Penicillium)、枝孢属(Cladosporium)、油瓶霉属(Lecythophora)、拟青霉属(Paecilomyces)、镰刀孢属(Fusarium)等。MIC检测发现有4株丝状真菌耐镉浓度较高, Paecilomyces lilacinus (Thom) Samson (6-20p), Penicillium pinophilum Hedgcock (6-16p), Penicillium rolfsii Thom (6-16m) 和Fusarium oxysporum Schlecht. (8-11p) 分别为200、40、25和15 mmol/L。【结论】从粤东北梅州市梅县铅锌尾矿废弃地分离到的72株丝状真菌, 不同程度耐受重金属镉, 在重金属污染的治理中有可能发挥作用。本研究为镉污染环境的微生物修复提供了重要菌株。
[Objective] The aim of this study is to isolate efficient cadmium-tolerant fungi from soils contaminated by heavy metals, and thereby to provide strains for heavy metals’ bioremediation. [Methods] Nine soil samples from Bingcun Pb-Zn mining waste land, Mei County, Guangdong Province, were used to isolate by dilution plate coating method with four different media. Strains were identi?ed by morphology and ITS rDNA sequence analyses. Minimum inhibitory concentration (MIC) for the isolated strains was also determined. [Results] Seventy-two cadmium-tolerant filamentous fungi belonging to the genera Aspergillus, Penicillium, Cladosporium, Lecythophora, Paecilomyces, Fusarium and others were isolated from the mining land. Four strains, i.e. Paecilomyces lilacinus (Thom) Samson (6-20p), Penicillium pinophilum Hedgcock (6-16p), Penicillium rolfsii Thom (6-16m) and Fusarium oxysporum Schlecht. (8-11p), were resistant to high cadmium concentrations of 200, 40, 25, and 15 mmol/L, respectively. [Conclusion] The strains isolated from contaminated soils showed minimum cadmium-tolerance to 1 mmol/L, thus they may play a role in the bioremediation of heavy metal pollution soils. The results imply the promising biosorption of cadmium-tolerant strains for bioremediation of heavy metals.
隆美容,谢小林,冯广达,姚青,朱红惠. 铅锌尾矿中耐重金属镉的丝状真菌的分离鉴定[J]. 微生物学通报, 2013, 40(12): 2203-2216
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