科微学术
微生物学通报
2025年4月4日 周五
国家自然科学基金面上项目(No. 31271870); 教育部高等学校博士学科点专项科研基金项目(No. 20113104110005); 上海市科委部分地方院校能力建设项目(No. 11310501100); 上海市科委工程中心建设项目(No. 11DZ2280300); 上海市科委科技创新行动计划项目(No. 12391901300)
TANG Xiao-Yang
1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China 2. Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China 3. Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, ChinaGUO Xiao-Bin
1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China 2. Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China 3. Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, ChinaGUO Dan-Feng
1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China 2. Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China 3. Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, ChinaHAN Ting
1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China 2. Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China 3. Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, ChinaXIE Jing
1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China 2. Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China 3. Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, ChinaPAN Ying-Jie
1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China 2. Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China 3. Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, ChinaZHAO Yong
1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China 2. Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China 3. Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China【目的】比较15 °C、20 °C和25 °C时, 9株不同致病性与非致病性副溶血性弧菌菌株在TSB (3% NaCl, pH 8.0)中的最大比生长速率之间的差异。【方法】应用Bioscreen C全自动微生物生长曲线分析仪测定副溶血性弧菌的最大比生长速率。【结果】15 °C、 20 °C和25 °C时, 9株副溶血性弧菌菌株间最大比生长速率的变异系数分别为20.72%、17.5%和15.98%。不同副溶血性弧菌最大比生长速率之间的差异随着温度的降低而增加。【结论】在进行定量微生物风险评估时, 应用仅基于一株菌建立的生长预测模型会给预测结果带来较大的不确定性。需建立可描述不同副溶血性弧菌菌株的最大比生长速率的随机模型来为定量微生物风险评估提供更准确有效的预测模型。
[Objective] Comparing the variability of the maximum specific growth rate, μmax, of 9 different pathogenic and non-pathogenic V. parahaemolyticus strains in TSB (3% NaCl, pH 8.0) at 15 °C, 20 °C and 25 °C. [Methods] An automated turbidimetric system, Bioscreen C, was used to determine the μmax of strains of V. parahaemolyticus. [Results] The coefficient of variation of μmax among 9 V. parahaemolyticus strains was 20.72%, 17.5% and 15.98% at 15 °C, 20 °C and 25 °C, respectively. The variability of μmax among different V. parahaemolyticus strains increased as temperature was decreased. [Conclusion] A quantitative microbial risk assessment of V. parahaemolyticus infection and illness based on the growth characteristics of a single strain of V. parahaemolyticus, introduces a bias in the assessment. It’s necessary to develop stochastic models as the more precise and effective predictive model to be utilized in quantitative microbial risk assessment.
唐晓阳,郭晓滨,郭丹凤,韩婷,谢晶,潘迎捷,赵勇. 高通量快速测定致病性及非致病性副溶血性弧菌最大比生长速率[J]. 微生物学通报, 2013, 40(11): 2138-2144
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