肉类腐败性假单胞菌群体感应信号分子的研究

Quantitation of quorum sensing signal molecules of Pseudomonas spp. isolated from meet

摘要:

【目的】研究两株假单胞菌的标准菌株荧光假单胞菌(Pseudomonas fluorescens)和铜绿假单胞菌(Pseudomonas aeruginosa)在纯培养条件下所释放的AHLs类信号分子种类、量和变化规律。【方法】利用乙酸乙酯等有机溶剂萃取菌种纯培养液的AHLs类信号分子, 检测手段利用HPLC-MS-MS。【结果】荧光假单胞菌释放信号分子的种类为: C4-HSL、C6-HSL、C8-HSL、3-oxo-C10-HSL、3-oxo-C12-HSL、3-oxo-C14-HSL。铜绿假单胞菌释放信号分子的种类为: C4-HSL、C6-HSL、C8-HSL、C10-SL、C12-HSL、C14-HSL、3-oxo-C8-HSL、3-oxo-C10-HSL、3-oxo-C12-HSL、3-oxo-C14-HSL。【结论】两株菌所释放各类信号分子的量均随时间变化, 当菌落数达到109?1010时信号分子的量达到峰值, 两株菌所释放各类信号分子含量差异较大。

Abstract:

[Objective] Different types, quantities and varying patterns of the AHLs released from pure cultured Pseudomonas fluorescens and Pseudomonas aeruginosa as the standard strains of Pseudomonas spp. were studied in this research. [Methods] AHLs in the pure culture solution were extracted via ethyl acetate and then identified through HPLC-MS-MS. [Results] The results showed that C4-HSL, C6-HSL, C8-HSL, 3-oxo-C10-HSL, 3-oxo-C12-HSL, 3-oxo-C14-HSL were the main signaling molecules released from Pseudomonas fluorescens while C4-HSL, C6-HSL, C8-HSL, C10-SL, C12-HSL, C14-HSL, 3-oxo-C8-HSL, 3-oxo-C10-HSL, 3-oxo-C12-HSL, 3-oxo-C14-HSL were the corresponding signaling molecules in Pseudomonas aeruginosa. [Conclusion] Quantities of signal molecules from the two strains were changing with time, when the number of colonies at 109?1010, reached the peak of the signal molecule. Also, significant differences of signaling molecules contents released from the 2 strains were found in this research.