西藏羊八井废弃热井沉积物中的真核微生物多样性

Eukaryotic microbes diversity in a disused thermal vent in Yangbajing, Tibet A. R.

摘要:

【目的】研究西藏羊八井废弃热井沉积物中的真核微生物多样性。【方法】采用ITS-rRNA基因分析法构建了沉积物中真核微生物的ITS-rRNA基因文库, 随机抽取文库中的克隆子进行扩增rDNA限制性酶切片段分析(ARDRA), 并对ITS-rRNA基因进行了系统发育分析。【结果】沉积物中主要的真核微生物有子囊菌、壶菌、担子菌、丝足虫、后生动物和非培养真菌。其中壶菌Chytridiales sp.、Physoderma maydis和Powellomyces sp., 子囊菌的假丝酵母(Candida parapsilosis)、鲁氏接合酵母(Zygosaccharomyces rouxii)和短梗霉(Aureobasidium pullulans)以及后生动物的轮虫(Lecane bulla)均未有报道存在于热泉或热井中。【结论】羊八井热井沉积物中具有丰富的真核微生物多样性。

Abstract:

[Objective] To discover the eukaryotic microbes diversity in sediment from a disused thermal vent in Yangbajing, Tibet A. R.. [Methods] To constructed the ITS-rRNA gene library of total DNA extracted from sediment by analyzed ITS-rRNA gene. Positive clones from the library were analyzed randomly by amplified ribosomal DNA restriction analysis (ARDRA). Phylogenetic trees based on eukaryotic ITS-rRNA gene sequences were built up by using MEGA 4.0 program. [Results] There were Ascomycota, Chytridiomycota, Basidiomycota, Cercozoan, Metazoan and uncultured fungus in sediment from Yangbajing thermal vent. Chytridiales sp., Physoderma maydis, Powellomyces sp., Candida parapsilosis, Zygosaccharomyces rouxii, Aureobasidium pullulans and Lecane bulla, all of them have not yet been reported to be inhabited in hot springs or thermal vents. [Conclusion] There were lots of eukaryotic microbes communities in sediment from the disused thermal vent in Yangbajing, Tibet A. R..