Aiming at the target to train the practical talents and the present status that general experimental accomplishment is rather low in local college, microbiology experiment teaching system was divided into three types: basis, synthetical advancing and research innovation. Gradual research-oriented teaching pattern was conducted by transition from students’ exploring guided by teacher to students’ exploring independently. The pattern prompted students to master the basic experimental operating technology and cultivated students’ ability of practice and comprehensive quality; synthetic and competency-based evaluation system was established to give an overall investigation on comprehensive ability of students and promote the teaching. In the system, both process and effect were taken into account, various evaluation subjects were involve.