高校学生宿舍室内空气中产超广谱β-内酰胺酶大肠埃希菌分离株ERIC-PCR指纹图谱分析

Analysis of ERIC-PCR fingerprints of extended-spectrum β-lactamase producing Escherichia coli in the air of college student’s dormitories

摘要:

【目的】了解大学生宿舍空气中产超广谱β-内酰胺酶(ESBLs)大肠埃希菌的检出率和分离株之间的遗传相似性, 为预防和控制传染病的传播提供依据。【方法】用FA-1型六级筛孔撞击式空气微生物采样器采集大学生宿舍内室内空气并分离鉴定其中的产ESBLs大肠埃希菌, 采用ERIC-PCR扩增基因组DNA, 形成聚类图谱, 分析分离株的相似性。【结果】从收集到的300份空气样品中分离鉴定出194株大肠埃希菌, 30株鉴定为超广谱β-内酰胺酶分离株, 检出率达15.46%; 产ESBLs大肠埃希菌ERIC-PCR指纹图谱条带相似性在50%?100%之间。【结论】大学生宿舍空气中存在着产ESBLs大肠埃希菌污染, 应加强对空气中大肠埃希菌耐药性监测。

Abstract:

[Objective] To investigate airborne extended-spectrum β-lactamase (ESBLs) producing Escherichia coli (E. coli) infection situation in the student dormitory in Chinese university and to analysis genetic similarities between different strains, and then provide a practical basis for preventing and controlling infectious diseases. [Methods] The air samples were collected by FA-1 six-stage microbial samplers in the different traditional student dormitory, E. coli strains were isolated and identified from the air samples, and then the enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) fingerprints of ESBLs-producing E. coli were analyzed. [Results] 194 strains of E. coli were isolated from 300 air samples, 30 strains of ESBLs-produing E. coli were founded and the resistant rate was 15.46%. The genetic similarity index of ESBLs-produing E. coli was 50%?100%. [Conclusion] The air in the student dormitory in university was contaminated by ESBLs-producing E. coli. The results stress the need for surveillance of the antibiotics resistance of E. coli in the air.