桃根癌病菌拮抗放线菌抑菌物质的分离纯化与结构鉴定

Identification of antimicrobial composition isolated and purified from antagonistic actinomycetes against Agrobacterium tumefaciens of peach

摘要:

【目的】为明确根癌拮抗放线菌G-19的抑菌活性物质。【方法】采用Sephadex LH-20柱层析、高效液相色谱及中压制备色谱等技术, 对桃根癌拮抗放线菌G-19发酵液中的抑菌活性成分进行分离纯化, 并通过LC-MS、NMR对其结构进行鉴定。【结果】分离得到化合物G-19-I的结构为邻苯二甲酸二丁酯(DBP), 化合物G-19-II的结构为2-(4-羟基苯基)-3,4-二氢-2H-苯并吡喃-3,4,5,7-四醇, 俗称无色天竺葵素。【结论】阐明了放线菌G-19抑菌活性成分的物质基础。

Abstract:

[Objective] To isolate and identify the antimicrobial active component extracted from fermentation broth of antagonistic actinomycetes strain G-19 against peach crown gall (Agrobacterium tumefaciens). [Methods] Sephadex LH-20 chromatography, HPLC and middle-pressure chromatography were used for isolation and purification. LC-MS, NMR were applied to identify structure of the active component. [Results] Compound G-19-I was isolated and structurally identified as 1,2-benzenedicarboxylic acid, 1,2-dibutyl ester (DBP); compound G-19-II was isolated and structurally identified as 2-(4-hydroxyphenyl)-3,4-dihydro- 2H-chromene-3,4,5,7-tetrol, commonly known as leucopelargonidin. [Conclusion] The material basis of the antibacterial constituents in antagonistic actinomycetes strain G-19 was further clarified.