南京市2011年乙型流感血凝素基因分子特征分析

Molecular characteristics of the hemagglutinin gene of influenza B viruses isolated in Nanjing, 2011

摘要:

【目的】分析2011年南京市乙型流感病毒的血凝素(HA)分子学特征。【方法】选择7株2011年南京市不同时间段有代表性的乙型流感毒株进行HA基因序列测定, 通过生物信息学方法对HA分子学特征进行分析。【结果】7株乙型流感毒株分为两个系, 4株为Victoria, 3株为Yamagata; 与2011年度疫苗株相比, Victoria和Yamagata系毒株分别在抗原位点146、197和116、198发生了氨基酸替换; 其中197和198位点分别是Victoria和Yamagata毒株的受体结合位点, 由于上述位点的替换使得Victoria系/Yamagata系毒株分别在197/196位增加了一个潜在的糖基化位点。【结论】2011年南京市乙型流感Victoria系和Yamagata系病毒同时存在, Victoria/Yamagata毒株197/198位点的氨基酸替换, 值得做进一步的探讨。

Abstract:

[Objective] To characterize the molecular characteristics of hemagglutinin (HA) of influenza B viruses isolated in Nanjing in 2011. [Methods] We chose 7 strains representative influenza B virus in different epidemic time in 2011. In order to monitor evolution; of the virus, then we carried out HA sequencing, and analyzing the genetic characteristics of HA with the bioinformatics method. [Results] Of the 7 randomly selected Inf-B strains, 4 strains were Victoria lineage strains, and 3 strains belong to Yamagata lineage. Comparison of nucleotide and amino acid sequences of HA gene with the correspondent vaccine strains, we find changes at antigenic sites 146 and 197 of Victoria lineage strains, and 116 and 198 of Yamagata lineage strains as well. 197/198 receptor-binding sites belong to Victoria/Yamagata lineage strains correspondently. As the result, 197/196 sites in Victoria/Yamagata isolates virus added an potential glycosylatio site. [Conclusion] Both B/Victoria and B/Yamagata lineage viruses co-circulated in Nanjing, the further researches of the changes at 197/198 sites of HA1 gene of Victoria/Yamagata isolates virus are valuable worthy.