海洋细菌SSQ500的鉴定及其发酵产物抗肿瘤作用机制研究

Identification of marine bacteria SSQ500 and the antitumor mechanism of fermentation products

摘要:

【目的】对具有抗肿瘤活性的海洋细菌SSQ500进行分类鉴定, 初步探讨其发酵产物的抗肿瘤作用机制。【方法】通过菌株的形态学观察、生理生化特征检测和16S rDNA序列分析及系统发育分析, 以确定菌株的分类。不同浓度的活性物质作用于肿瘤细胞不同时间后收集细胞, 流式细胞术分析人肝癌细胞(SMMC-7721)凋亡比例, 检测Caspase3、Caspase8蛋白的表达。【结果】初步鉴定菌株SSQ500属于粘球菌属(Myxococcaceae sp.)。该菌株发酵产物能诱导SMMC-7721凋亡, 上调Caspase3、Caspase8蛋白的表达。【结论】SSQ500属于粘球菌属, 其发酵产物的抗肿瘤作用机制为诱导肿瘤细胞凋亡, 可能与上调Caspase3、Caspase8蛋白的表达有关。

Abstract:

[Objective] To identify the marine bacteria SSQ500 with antitumor activity, and to study the antitumor mechanism of fermentation products. [Methods] The strain SSQ500 was identified using its morphological characteristics, physiological, biochemical properties and 16S rDNA gene sequence. The tumor cell was collected after different concentration activity compounds acted on difference time. The inverse proportion of apoptosis of the liver cancer cell SMMC-7721 was analyzed by flow cytometry. To study the protein expression by examine Caspase3, Caspase8. [Results] The strain SSQ500 was identified belong to Myxococcaceae sp.. The activity compound SSQ produced by this bacteria can induce SMMC-7721 cell to apoptosis by up-regulate the expressions of Caspase3, Caspase8. [Conclusion] The SSQ500 belongs to Myxococcaceae sp., and the fermentation product of this bacteria can induce tumor cell to apoptosis. That may be related to up-regulate the expressions of Caspase3, Caspase8.