矿泉水和山泉水中粪链球菌污染调查及主要污染菌株的ERIC-PCR分型研究

Studies on the contamination investigation and ERIC-PCR typing of Enterococcus faecalis in mineral and spring water

摘要:

【目的】通过调查广东省矿泉水和山泉水生产企业水源水、碳后水和成品水中的粪链球菌(Enterococcus faecalis)污染情况, 为生产企业微生物控制提供相应的依据。【方法】粪链球菌的检测方法采用稍作修改的GB/T8538-2008/4.53, 并运用ERIC-PCR技术对主要污染菌株进行分型。【结果】206份水样中有35份水样检出粪链球菌, 其中水源水20份、碳后水13份和成品水2份, 水源水、碳后水和成品水的污染率分别为26.3%、20%和3.1%, 总污染率为17%。矿泉水和山泉水的总污染率分别为3.8%和25.2%, 山泉水、地下水和地表水的水源污染率分别为33.3%和63.6%。ERIC-PCR指纹图谱聚类分析显示35株菌分为3簇, 主要污染菌基因型在B簇。【结论】广东省山泉水的粪链球菌污染率明显高于矿泉水的污染率, 同时山泉水的水源水污染率中, 地表水高于地下水。

Abstract:

[Objective] The contamination of Enterococcus faecalis in source water, activated carbon filtered water and finished water from mineral and spring water plants in Guangdong province was investigated for offering important data to control the E. faecalis pollution. [Methods] The standard GB/T8538-2008/4.53 with little modification was used to detect the existence of E. faecalis, then to carry out the ERIC-PCR fingerprinting of E. faecalis. [Results] Thirty-five samples were detected positively for E. faecalis with the total contamination rate of 17%. Among the 35 positive samples, the source water, activated carbon filtered water, and finished water samples were 20 (26.3%), 13 (20%) and 2 (3.1%) respectively. According to ERIC-PCR, E. faecalis strains from 35 samples were divided into three groups and the type B was the main genotype in Guangdong province. [Conclusion] The results indicated that the contamination rate of E. faecalis in spring water in Guangdong province was much higher than that of mineral water, moreover, as far as spring water was concerned, the source water contamination rate was higher from surface water than from ground water.