云南大理烟区烟叶内生真菌多样性及分布特征

Diversity and distribution characteristics of endophytic fungi in Nicotiana tabacum in Dali District, Yunnan Province

摘要:

【目的】研究大理烟区烟叶内生真菌的多样性及分布特征。【方法】通过形态特征和ITS-rDNA序列分析对获得的菌株进行分类。【结果】从烟草叶片中共分离得到内生真菌1 568株, 分别归于31个属, 在属水平上的优势菌群为链格孢属(Alternaria)和镰孢属(Fusarium), 分别占真菌总数的40.79%和18.73%。【结论】烟草内生真菌的数量、种类、多样性和分布分别在不同部位叶片间和不同地区间存在差异, 在下部叶数量较多, 而上部叶的种类较多, 多样性更丰富。随着烟草的发育成熟, 内生真菌数量、种类和多样性增加, 在现蕾期和成熟期最大。

Abstract:

[Objective] Researched the diversity and distribution characteristics of endophytic fungi in Nicotiana tabacum in Dali District. [Methods] These strains were identified into genera based on morphological characteristics combined wtih ITS-rDNA gene sequences. [Results] A total of 1 586 strains of endophytic fungi were isolated from the leaves of Nicotiana tabacum and were identified into 31 genera. Alternaria and Fusarium are dominant fungal endophytes, occupying 40.79% and 18.73%, of total isolated strains respectively. [Conclusion] The quantity, diversity and distribution of the endophytic fungi were varied from different part leaves and different districts of N. tabacum. Larger quantity of endophytic fungi was found in lower part leaves than that of the upper ones. Otherwise, richer diversities of endophytic fungi were found in upper leaves. Along with the development of N. tabacum, the richness and diversity of endophytic fungi in N. tabacum leaves were increased and reached the largest during the squaring stage and maturity stage.