木霉属中国新纪录种Trichoderma pleuroticola和T. pleurotum

Two new Chinese record of the genus Trichoderma: Trichoderma pleuroticola and T. pleurotum

摘要:

【目的】对蔬菜大棚土壤中和阿魏菇腐烂的菌盖上分离的两株木霉菌进行分类鉴定。【方法】结合形态学分类特征和ITS序列分析的方法进行鉴定。【结果】从蔬菜大棚的土壤中和阿魏菇腐烂的菌盖上分离的两株木霉菌分别为Trichoderma pleuroticola和T. pleurotum。T. pleuroticola的形态特征与T. harzianum相似, 但其分生孢子显著大于T. harzianum的分生孢子, 且在PDA上产生黑褐色的色素以及黄色的结晶物。T. pleurotum典型特征是分生孢子梗单生, 有时匍匐, 分枝散生, 初级分枝和分生孢子梗顶端聚生, 类似粘帚霉。【结论】分离的两株木霉分别是T. pleuroticola和T. pleurotum, 为木霉菌中国新纪录种。

Abstract:

[Objective] Identification of two Trichoderma isolates were isolated from the soil in vegetable greenhouses and the pileus of Asafoetida mushroom. [Methods] By combination of morphological charaters and application of internal transcribed spacer (ITS). [Results] Two Trichoderma isolates were identified as Trichoderma pleuroticola S.H.Yu & Park sp. nov. and Trichoderma pleurotum S.H.Yu & Park. The morphological charaters of T. pleuroticola is similar with T. harzianum, but its conidiospore is obviously more than T. harzianum, secretes dark brown pigment, and forms yellow crystal on PDA medium. The typical characteristics of Trichoderma pleurotum is that its conidiophores are mostly solitary and more or less prostrate, branches scattered, arising separately and bearing crowded whorls of appressed phialides at the apex rsembling the conidiophore in Gliocladium. [Conclusion] Two Trichoderma isolates are T. pleuroticola and T. pleurotum respectively, which are two new record species in China.