Abstract:[Objective] The objective of this study was to examine taxonomic position of two Trichoderma strains which were isolated from soil in southern China. [Methods] PDAm was used as a selective medium for isolating Trichoderma, then two strain was indetified by analysis of internal transcribed spacer regions of the rRNA gene cluster (ITS), partial sequences of transcription extensions factor 1-alpha (tef1-α) and observation of morphological characters. For phylogenetic analyses in MEGA 4.0, both ITS and tef1-α gene sequences were analyzed using the maximum parsimony approach of close-neighbor-interchange algorithm with search level 3, in which the ini-tial trees were obtained with the random addition of sequences (1 000 replicates). [Results] The ITS gene sequences of the strain CM01 were naturally clustered with ITS gene sequences of Trichoderma intricatum strain GJS 02-78 in GenBank with 99% of homology, and had the closest phylogenetic relationship with T. intricatum GJS 02-78, Trichoderma atroviride DAOM 179514 in phylogenetic tree which based on ITS sequences of strains; the tef1-α gene sequence of the strain CM01 were naturally clustered with Hypocrea intricate strain GJS 02-78 are the closest phyloge-netic relatives with 99% of homology, and had the closest phylogenetic relationship with H. intri-cata GJS 02-78 in phylogenetic tree which based on tef1-α sequences of strains; its morphological description accorded with type strain’s. Meantime, the ITS gene sequences of the strain SCGA5003 were naturally clustered with ITS gene sequences of Trichoderma stromaticum strain GJS 97-181 in GenBank with 99% of homology, and had the closest phylogenetic relationship with T. stromaticum GJS 97-179, GJS 97-180, GJS 97-181, GJS 97-182, GJS 97-183 in phyloge-netic tree which based on ITS sequences of strains; the tef1-α gene sequence of the strain SCGA5003 were naturally clustered with T. stromaticum strain GJS 97-183 in GenBank with 94% of homology, and had closest relationship with T. stromaticum CQSQ1032, GXNN7006 in phy-logenetic tree which based on tef1-α sequences of strains; its morphological description were con-sistent with type strain’s. [Conclusion] Utilizing a comprehensive approaches of morphological characters and molecular identification, we concluded that we found two new Chinese record spe-cies of the genus Trichoderma, strain CM01 was identified as Trichoderma intricatum Samuels & Dodd/Hypocrea intricata Samuels et Dodd, strain SCGA5003 was identified as Trichoderma stromaticum Samuels & Pado-Schulth/Hypocrea stromatica Bezerra, Costa & Bastos.
