Abstract:[Objective] study on yeast and yeast-like collected from the Rumex hastatus in five regions (Jinning, Xiangyun, Chenghai, Luguhu and Erhai) of Yunnan province. [Methods] The yeasts and yeast-like were isolated by spreading plate and identified by using large-subunit 26S rDNA gene D1/D2 domain sequence analysis and morphological. The ability of the yeast strains to produced extracellular enzyme and lipid were tested by screening media and Sudan black-B staining method. [Results] 82 yeasts and 99 yeast-like strains were isolated from the Rumex hastatus in five regions. The yeast strains were identified as belonging to 6 genera and 16 species and 1 suspected new species. And the yeast-like strains were identified as 1 species and 3 variety. The dominant genera in Rumex hastatus flowers were yeast-like Aureobasidium, followed by Rhodotorula and Cryptococcus. It has been obtained that 134 strains produced extracellular enzyme and 83 strains produced lipid. [Conclusion] The diversity of yeasts and yeast-like species from Rumex hastatus flowers in five regions are more abundant. With characteristics of producing amylase, protease, cellulase, lipase and lipid, these strains will have a potential value of application.
