云南省德宏州含羞草β-根瘤菌多样性及系统发育研究

b-Rhizobia associated with Mimosa spp. from Dehong district of Yunnan province analyzed by 16S rDNA PCR-RFLP and SDS-PAGE

摘要:

【目的】通过对分离自云南德宏州的含羞草β-根瘤菌进行遗传与表型多样性研究, 揭示我国含羞草β-根瘤菌的物种多样性。【方法】应用16S rDNA PCR-RFLP、全细胞蛋白SDS-PAGE电泳及16S rDNA全序列分析对分离得到的60株含羞草根瘤菌进行多样性研究。【结果】16S rDNA PCR-RFLP及全细胞蛋白SDS-PAGE图谱分析将供试菌株分为2个遗传型群和2个表型群, 分别与贪铜菌属(Cupriavidus)和伯克霍尔德菌属(Burkholderia)参比菌株聚群。经16S rDNA全序列分析, 供试菌株被归到台湾贪铜菌(Cupriavidus taiwanensis)、含羞草伯克霍尔德菌(Burkholderia mimosarum)及结瘤伯克霍尔德菌(Burkholderia phymatum)等3个种群。【结论】云南德宏州的含羞草β-根瘤菌主要为贪铜菌及伯克霍尔德菌类群, 其中贪铜菌占绝对优势, 且存在遗传和表型的丰富多样性, 该研究揭示了含羞草β-根瘤菌的物种多样性并丰富了我国β-根瘤菌菌种资源。

Abstract:

[Objective] Genetic and phenotypic diversity of β-rhizobia associated with Mimasa spp. in Dehong district of Yunnan province were performed to revealed the diversity of rhizobial resources in China. [Methods] 60 isolates were analyzed by 16S rDNA PCR-RFLP, total cell protein SDS-PAGE analysis and 16S rDNA sequencing. [Results] All the isolates were clustered two genetic groups and two phena groups respectively, by characterizing 16S rDNA PCR-RFLP and SDS-PAGE anlaysis. The strains in each group are in coinciding with between two methods. All strains clustered with type strain of Cupriavidus, and Burkholderia. From the phylogenetic tree of 16S rDNA sequence, the test strains were belonged to the species of Cupriavidus taiwanensis, Burkholderia mimosarum and Burkholderia phymatum. [Conclusion] At present study, we find that β-rhizobium from Dehong district were belonged to Cupriavidus and Burkholderia mainly. The Cupriavidus spp. were predominated in these strains. These records showed the diversity of Rhizobium from Mimosa spp. and enriching resources of β-rhizobium in China.