傅立叶变换红外光谱技术对5种沙门氏菌的快速分类鉴定

Rapid differentiation and identification of five species of Salmonella by FT-IR spectroscopy

摘要:

【目的】建立沙门氏菌属内鼠伤寒沙门氏菌、肠炎沙门氏菌、猪霍乱沙门氏菌、亚利桑那沙门氏菌、波斯坦沙门氏菌5种菌的傅立叶变换红外(Fourier transform infrared, FT-IR)光谱数据库及FT-IR分类鉴定方法。【方法】应用FT-IR技术对5种沙门氏菌进行指纹图谱数据采集, 应用化学计量学分析方法对光谱进行分析。【结果】建立了5种沙门氏菌的标准FT-IR光谱数据库, 用于FT-IR技术对5种可疑目标沙门氏菌进行鉴定; 建立了基于主成分分析(Principal component analysis, PCA)和分级聚类分析(Hierarchical cluster analysis, HCA)两种聚类分析模型, 均可成功将5种沙门氏菌进行区分。【结论】傅立叶变换红外光谱分析方法简便、快速、易操作, 结果重现性好, 是一种区分5种沙门氏菌的有效方法。

Abstract:

[Objective] To establish a standard fourier transform infrared (FT-IR) spectral library and a FT-IR method of differentiation and identification of five species of Salmonella, viz Salmonella typhimurium, Salmonella enteritidis, Salmonella choleraesuis, Salmonella arizonae and Salmonella potsdam. [Methods] FT-IR fingerprint absorption spectra of five species of Salmonella were collected and analyzed by chemometric methods. [Results] A standard FT-IR spectral library was created and could be used to compare with those of the target Salmonella and identify them; two cluster models of principal component analysis (PCA) and hierarchical cluster analysis (HCA) were established and could differentiate and identify well the five species of Salmonella. [Conclusion] As a rapid, easy-to-use and accurate technique, FT-IR spectroscopy is an effective tool to differentiate and identify five species of Salmonella studied here.