Abstract:We reformed teaching contents, teaching means, teaching methods and evaluation methods of food microbiology experimental teaching in order to construct three-dimensional experimental teaching system which contained Basic experiment—Integrated improve experiment—Innovative experiment, so that it could possess systematic teaching, hierarchical courses and multiform contents. And it had trained the students well and improved their special skills. Good effects had been made since this system was very helpful to improve their consciousness of innovation and integrative abilities in doing experiments.