ATP生物发光测定试剂研究进展

Reserach progress on ATP bioluminescence reagent

摘要:

萤火虫荧光素酶是ATP生物发光试剂的关键组成部分, 可通过萤火虫尾提取纯化或基因工程技术制备, 酶的活力和纯度决定了ATP生物发光试剂的性能。迄今许多先进技术在ATP生物发光试剂的制备中均有应用, 包括酶基因工程改造技术、ATP循环的酶法放大技术、荧光素酶蛋白的活力及发光稳定技术, 特异的细胞ATP提取技术等。ATP生物发光试剂的研究焦点主要集中在提高发光试剂的检测灵敏度和性能、增加产品的适应性等方面。

Abstract:

Firefly luciferase is the key component of ATP bioluminescence reagent, gained from firefly lantern throuh extraction and purification or preparation through genetic engineering, the performance of ATP bioluminescence reagent was decided by the vitality and the purity of firefly luciferase. Up to now, many present advanced technology were applied on preparation the reagent such as genetic engineering, ATP amplification device, stabilization technology of luciferase protein and luminescence, and so on. Now research focus on improving detection sensitivity and luminescence performance of the ATP bioluminescence reagents, further raising the adaptability of ATP bioluminescence reagents.