泰安枣疯病植原体的分子鉴定

Molecular identification of jujube witches’-broom phytoplasmas strains from Tai’an

摘要:

【目的】对3个枣疯病病原物泰安株系进行分子鉴定。【方法】采用植原体通用引物对R16F2n/R16R2, 通过直接PCR技术, 扩增枣疯病植原体16S rDNA基因, 通过16S rDNA基因序列分析和在线模拟16S rDNA-RFLP分析, 并将其16S rDNA基因序列提交到GenBank数据库。【结果】3个枣疯病病原物16S rDNA基因片段与16Sr Ⅴ-B亚组中枣疯病植原体(AB052876和AF279272)、樱桃致死黄化植原体(AY197659)及杏卷叶植原体(FJ572660)的同源性高达99.5%?99.7%, 分别命名为枣疯病植原体泰安圆铃1号株系(Jujube witches’-broom phytoplasma strain Yuanling1, JWB-Yuanling1, TA)、枣疯病植原体泰安鲁北冬枣株系(Jujube witches’-broom phytoplasma strain Lubeidongzao, JWB-Lubeidongzao, TA)和枣疯病植原体泰安大白铃株系(Jujube witches’-broom phytoplasma strain Dabailing, JWB-Dabailing, TA), 基因登录号分别为: HM989946、HM989947和HM989948。【结论】3个枣疯病植原体泰安株系均归属于16Sr Ⅴ-B亚组。

Abstract:

[Objective] To identify the three jujube witches’-broom phytoplasmas strains from Tai’an. [Methods] The phytoplasmas strains were identified by 16S rDNA sequence analysis via PCR amplification of 16S rDNA gene with the universal primer pair of R16F2n/R16R2 for phytoplasmas and online virtual 16S rDNA-RFLP analysis, and then their 16S rDNA sequences were deposited in GenBank. [Results] The results showed that the 16S rDNA sequences of the three strains shared the identity of 99.5%?99.7% with that of jujube witches’-broom phytoplasmas (AB052876 and AF279272), cherry lethal yellows phytoplasmas (AY197659) and apricot leaf roll phytoplasmas (FJ572660) which belong to 16Sr Ⅴ-B subgroup, and they were named as jujube witches’-broom phytoplasma strain Yuanling1 (JWB-Yuanling1, TA), jujube witches’-broom phytoplasma strain Lubeidongzao (JWB-Lubeidongzao, TA) and jujube witches’-broom phytoplasma strain Dabailing (JWB-Dabailing, TA) respectively. The 16S rDNA sequences of three JWB strains were deposited in GenBank with the accession numbers of HM989946, HM989947 and HM989948 respectively. [Conclusion] The three phytoplasmas strains from Tai’an were identified as the members of 16Sr Ⅴ-B subgroup.