白腐菌协同热水预处理对毛白杨化学组分和酶水解效果的影响

Influences of combination of biological pretreatment with liquid hot water pretreatment on chemical components and enzymatic hydrolysis of Populus tomentosa

摘要:

【目的】增强真菌预处理的效率和降低热水预处理对反应条件的要求。【方法】综合利用白腐菌和热水预处理毛白杨, 分析此方法对毛白杨化学组分和酶水解效果的影响。【结果】白腐菌 Lenzites betulinus C5617协同热水处理, 损失率最高达70.70%。纤维素在2个预处理阶段都有损失, 其中L. betulinus C5617达到29.62%。木质素的降解主要集中在白腐菌预处理阶段, 其中L. betulinus C5617降解的酸不溶木素较多, 达到了16.98%。综合预处理显著改善了毛白杨的酶水解效果。与只经热水预处理的样品相比较, L. betulinus C5617和P. sanguineus D9497协同热水处理分别引起还原糖得率上升了20.60%和12.23%。【结论】综合预处理降低了热水解对反应条件的要求, 节约了预处理成本。

Abstract:

[Objective] Enhance the efficiency of fungal pretreatment and lower the severity of liquid hot water (LHW) pretreatment. [Methods] Combinations of white rot fungi and LHW were employed to pretreat Populus tomentosa, the influences of this combined pretreatments on the chemical components and enzymatic hydrolysis of P. tomentosa were studied. [Results] The highest hemicellulose removal of 70.70% was observed by combination of Lenzites betulinus C5617 with LHW pretreatment. Celluloses were degraded in both step of the combined pretreatments, combination of L. betulinus C5617 with LHW caused the highest cellulose loss of 29.62%. Lignin loss happened mostly in the step of fungal pretreatment, and L. betulinus C5617 caused a higher degradation (16.98%) of acid insoluble lignin among the two fungi. The combined pretreatments improved the enzymatic hydrolysis of P. tomentosa significantly. Compared with sole LHW treatment, combination of L. betulinus C5617 with LHW pretreatment resulted in an increase by 20.60%, and an increase (12.23%) of reducing sugar yield were obtained by combination of P. sanguineus D9497 with LHW treatment. [Conclusion] This combined pretreatment can lower the severity of LHW pretreatment and was cost-effective.