Abstract:To enhance the science knowledge base of the students majored in bio-engineering and maximize the effect of microbiology course in professional basic courses to make the students grasp the knowledge firmly and cultivate their many capacities at the same time, we developed a method called “Five-step Teaching” which follows “guiding-study—self-study— teacher-tutor—reciprocal study—whole-class-lecture” to reform the education mode. The results indicated: (1) The students not only grasped the knowledge firmly but also changed passive learning into active learning through “Five-step Teaching” practice. (2) As a result of self-taught, preparation, presentations, evaluation and so on, students’ self-confidence, awareness of competence as well as the abilities of logical thinking, understanding, generalizing and language expression and so on were also developed. (3) Score evaluation system would be more reasonable by virtue of “Five-step Teaching” practice.