一株苯酚降解菌的鉴定及其降解特性

Identification of degradative bacterium of phenol and its degradation characterization

摘要:

【目的】鉴定从某化工厂附近土样中分离到的一株耐高浓度苯酚的菌株T10, 通过优化菌株的培养条件提高菌株对苯酚的降解率。【方法】根据菌株的形态、生理生化鉴定及16S rDNA测序分析确定其种属, 以液体摇瓶培养菌株T10对苯酚的降解率为指标, 对菌株的生长条件进行优化。【结果】菌株T10属恶臭假单胞菌(Pseudomonas putida)。添加葡萄糖、蛋白胨能有效缩短T10菌的生长周期, 并使苯酚的降解率提高1.7倍。在菌体初始接种浓度为10%、温度为30 °C、转速为180 r/min条件下, 对初始苯酚浓度、pH和装液量的响应面优化结果如下: 初始苯酚浓度3 000 mg/L、pH 7.5和装液量80?mL/250 mL, 苯酚去除率最高可达到87.56%。【结论】T10菌能够耐受较高浓度的含酚废水, 并且对苯酚有较强的降解能力, 为下一步利用生物法处理含酚废水提供科学依据。

Abstract:

[Objective] A phenol-degradative strain named T10 which was isolated from the soil sample of a chemical plant was identified, and increased degradative rate of phenol by optimizing the growth conditions. [Methods] The strain was identified by morphological observation, physiological and biochemical properties and 16S rDNA sequence analysis. The phenol-degradative capacity of strain T10 was analyzed when they were cultured in a series of liquid media containing different pH, initial phenol concentration and liquid volume. [Results] The strain T10 belongs to Pseudomonas putida. Glucose and peptone can reduce growth period of strain T10, and the degradation rate of phenol was improved 1.7 times. Under the condition of inoculating concentration 10%, 30 °C and 180 r/min, the phenol removal rate of strain T10 could reach 87.56% when initial phenol concentration, pH and liquid volume were 3?000?mg/L, 7.5, 80 mL/250 mL, respectively. [Conclusion] The experimental data show that strain T10 can tolerate wastewater containing higher concentration phenol, and has stronger degradation ability on phenol. These results would provide reliable basis for further treatment of wastewater containing phenol by the means of biological method.