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微生物学通报

盐生植物翅碱蓬的内生真菌多样性分析
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沈阳农业大学青年科学基金项目


Diversity of endophytic fungi of Suaeda heteroptera Kitag.
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    摘要:

    【目的】对辽宁省盘锦市双台子河口国家自然保护区的盐生植物翅碱蓬(Suaeda heteroptera Kitag.)的内生真菌进行研究。【方法】利用传统分离方法对翅碱蓬的根、茎、叶组织进行分离和纯化, 根据形态学和理化特征, 并结合rDNA-ITS序列分析对菌种进行鉴定。【结果】共分离得到内生真菌49株, 这些内生真菌分属于13个属, 其中小丛壳菌属(Glomerella)、链格孢属(Alternaria)、毛盘孢属(Colletotrichum)和枝孢属(Cladosporium)为优势菌属。翅碱蓬的内生真菌分布具有一定的组织特异性, 两种不同生境中的翅碱蓬内生真菌群落丰富度差异较小, 相似度较高, 但在某些种属上存在特异性。【结论】翅碱蓬体内含有丰富的内生真菌资源, 其内生真菌具有很高的宿主特异性, 而且其分布受生境影响。

    Abstract:

    [Objective] The populations of endophytic fungi from halophyte Suaeda heteroptera Kitag. grown in the salt flats in Shuangtaizi Estuary of Panjin, Liaoning province, China, were investigated. [Methods] Endophytic fungi were isolated and purified from the roots, stems and leaves of Suaeda heteroptera Kitag. with traditional method. Isolates were identified based on morphological, physiological and biochemical characteristics and rDNA-ITS sequence analysis. [Results] Overall, 49 endophytic fungi strains were isolated and purified, these strains were classified into 13 different genera, in which Glomerella, Alternaria, Colletotrichum and Cladosporium were dominant genera. The distribution of endophytes of host plants have some degree of tissue preference, and though there was not significant difference of the abundance level in different habitats, habitat specificity of some endophytic fungi communities was presented. [Conclusion] Our results suggested that Suaeda heteroptera Kitag. have abundant resouces of endophytic fungi, the species diversity of endophytic fungi communities is affected both by its host preference and by its different habitats.

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钮旭光,宋立超,韩梅,肖亦农. 盐生植物翅碱蓬的内生真菌多样性分析[J]. 微生物学通报, 2012, 39(10): 1388-1395

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  • 在线发布日期: 2012-10-24
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