裸盖菇和斑褶菇属真菌菌种的有效分离方法

The effective method of fungal strain isolation for the genera Psilocybe and Panaeolus

摘要:

【目的】探讨获取裸盖菇属及斑褶菇属真菌纯培养的有效分离方法。【方法】采用菌褶接种法和孢子弹射法进行分离, 以形态鉴定为基础, 通过ITS区测序并与DNA序列库中已知序列进行比对的分子鉴定方法鉴别分离培养物的真伪, 以确定分离方法的可靠性。【结果】对采自云南的28个裸盖菇属和斑褶菇属菌株进行了分离, 菌褶接种法有24个菌株分离纯化成功, 成功率达86%, 而孢子弹射法仅有7个菌株分离成功, 成功率为25%。【结论】菌褶接种法对于裸盖菇属和斑褶菇属真菌是一种有效而简便易行的分离方法, 该法利用菌褶为产孢组织的优势, 无需对菌褶进行表面消毒, 易于纯化成功, 值得在其他类似的腐生小型薄盖伞菌类群的分离中尝试应用。

Abstract:

[Objective] To explore an effective method for accurately getting fungal strain isolates from the genus Psilocybe and Panaeolus. [Methods] Twenty-eight freshly collected fruit bodies were used as study materials. The cultures isolated from gill and basdiospores were identified based on morphology and sequence of ITS rDNA. [Results] Twenty-four isolates (86%) were purified successfully by gill inoculation method and seven (25%) by basidiospore discharge. [Conclusion] Gill is the spore-bearing tissue of agarics, and easy to germinate. Furthermore it is no need to sterilize the surface of the gill for purification in culture medium. This isolation approach may be extended in other groups of the saprophytic fungi characterized by small fruiting body size and thin pileus, similar to these two genera.