不同预处理条件对海水养殖废弃物发酵产氢的影响

Effects of different pretreatments on hydrogen production from mariculture organic waste

摘要:

【目的】利用海水养殖场有机废弃物厌氧发酵产氢, 可在减少有机污染物的同时获取氢气。【方法】以海水养殖场有机废弃物为底物, 比较嗜热酶(S-TE)、酸、碱、灭菌、微波不同预处理方法对厌氧发酵产氢效果的影响, 并对发酵过程中底物性质变化[SCOD、可溶性蛋白质、可溶性糖、pH、VFAs (挥发性脂肪酸)和乙醇]进行探讨。【结果】灭菌预处理产氢效果最好, 产氢率为22.0 mL/g VSS, 酸处理的效果最差, 产氢率为7.6 mL/g VSS。可溶性糖大量消耗之后, 氢气不再产生。接种S-TE预处理污泥的底物能更多地释放营养物质, 并在整个发酵过程中保持较为稳定的pH值。发酵过程中产生的VFAs主要成分是乙酸, 在发酵后期出现乙醇。【结论】灭菌预处理是海水养殖场有机废弃物厌氧发酵产氢的最佳预处理方法, 可溶性糖为这一过程主要的营养来源。

Abstract:

[Objective] The utilization of using mariculture organic waste for anaerobic hydrogen fermentation could reduce organic pollutant and obtaining clean energy hydrogen simultaneously. [Methods] We used mariculture organic waste for hydrogen production, and investigated the effect of different pretreated methods of, solubilization by thermophilic enzyme (S-TE), acid, alkaline, sterilized, and microwave on mariculture organic waste hydrogen production. The efficiency of these pretreatments and profiles of SCOD, soluble protein and carbohydrate, pH value, VFAs and ethanol during the fermentation were also monitored. [Results] The maximum hydrogen yield was 22.0 mL/g VSS, which was obtained from sterilized pretreatment, whereas the minimum hydrogen yield of 7.6 mL/g VSS was obtained from acid pretreatment. The fermentation stopped after soluble carbohydrate was largely consumed. More nutrients could be released from mariculture organic waste after S-TE pretreatment than other pretreatments, and the pH value was remained stable during the fermentation. The main composition of VFAs was acetic acid, and ethanol was produced at the end of fermentation. [Conclusion] Sterilized pretreatment was the optimum pretreatment method of anaerobic hydrogen fermentation from mariculture organic waste, and soluble carbohydrate was the main nutrient resource during the fermentation.