一株引起马来甜龙竹组培污染内生菌的分离与鉴定

Isolation and identification of pollution endophyte during tissue culture of Dendrocalamus aspera

摘要:

【目的】对一株引起马来甜龙竹组培污染内生菌的分离与鉴定。【方法】采用改良的NA培养基分离纯化菌株, 并通过菌体的形态结构观察、生理生化试验及其16S rDNA序列同源性分析对其进行鉴定。【结果】菌株SWFU01的形态特征及生理生化试验结果与解淀粉芽孢杆菌[Bacillus amyloliquefaciens (Fukumoto) Priest et al.]的描述基本相同; 16S rDNA序列分析表明, 该菌株与解淀粉芽孢杆菌JS在同一系统发育分支, 其同源性为99.28%。【结论】综合形态学特征、生理生化特征以及16S rDNA序列分析的研究结果, 菌株SWFU01被鉴定为解淀粉芽孢杆菌。

Abstract:

[Objective] To isolate and identify the endogenous bacteria, which caused tissue culture contamination of Dendrocalamus aspera. [Methods] Using the improved NA culture medium to isolate and identify the strain, then identify the bacterial species by morphology observation, physiological and biochemical tests and the sequence analysis of the 16S rDNA. [Results] The morphological characteristics of the strain SWFU01, together with related physiological and biochemical tests, match the descriptions of Bacillus amyloliquefaciens (Fukumoto) Priest et al.. The sequence analysis of 16SrDNA indicates that this strain has the same embranchment with the Bacillus amyloliquefaciens JS in the Phylogenetic tree, the homology is 99.28%. [Conclusion] By combining the traditional morphology observation and physiological and biochemical tests, the SWFU01 strain is identified as Bacillus amylolique-faciens.