黄曲霉毒素B1脱毒菌株LAB-10的分离、鉴定及降解能力分析

Isolation, identification and bio-detoxification analysis of an aflatoxin B1 degradative strain LAB-10

摘要:

【目的】从乳酸菌群含量丰富的动物肠道中筛选具有黄曲霉毒素B1 (AFB1)脱毒应用前景的乳酸菌种。【方法】通过设计脱毒乳酸菌种特异性富集分离培养基, 从肉鸡肠道粪便中筛选具有AFB1脱毒能力的微生物菌种, 对脱毒菌种进行脱毒机理初步分析, 并通过形态学、生理生化和系统发育学方法, 鉴定脱毒菌种的系统分类学地位。【结果】脱毒菌株LAB-10经脱毒机理及降解能力测试分析, 初步确定其AFB1的脱毒机理为生物降解作用, 菌株LAB-10对14 μg/L 浓度AFB1在48 h的降解率为63.4%。形态学、生理生化及系统发育学研究结果表明, 菌株LAB-10系统分类学地位为乳酸杆菌属的发酵乳杆菌。【结论】发酵乳杆菌LAB-10属于益生菌群, 生物安全性高, 该菌株在黄曲霉毒素降解测试培养基中显示出显著的AFB1降解能力, 具有一定的应用潜力。

Abstract:

[Objective] Lactic acid bacterium with aflatoxin B1 detoxification abilities was isolated and evaluated from chicken gut for the present study. [Methods] A special enrichment and isolation technology was designed for aflatoxin B1 detoxified lactic acid bacteria, and the aflatoxin B1 detoxification strain was further determined for its taxonomical position using morphological, physiological and biochemical characteristics combined with phylogenetic analysis. [Results] A lactic acid bacterium strain, which designated LAB-10, displayed aflatoxin B1 detoxification ability was isolated by the present technology. The detoxification analysis results displayed that strain LAB-10 was a aflatoxin B1 degradative bacterium and there was 63.4% of aflatoxin B1 could be decomposed by the strain under the concentration of 14 μg/L, within 48 hours. On the base of taxonomical analysis results, the new isolated strain LAB-10 was identified as Lactobacillus fermentum. [Conclusion] The isolate LAB-10 of the present study was Lactobacillus fermentum and the bio-safety characteristics implied that the degradative stain has potential values for the application of aflatoxins detoxification.