新疆阿克苏地区盐碱地细菌类群多样性及优势菌群分析

Analysis of the bacterial diversity and dominant population in Akesu Saline-alkali in Xinjiang

摘要:

【目的】研究新疆阿克苏地区盐碱土样中细菌类群多样性和优势种群, 及其与环境因子的相关性。【方法】采用基于16S rDNA 的变性梯度凝胶电泳(Denaturing gradient gel electrophoresis, DGGE)、克隆测序和典型相关性分析(CCA)的方法。【结果】优势菌群序列分析表明有29个序列属于未培养的微生物, 其他43个序列分属细菌的9个目: 粘球菌目(Myxococcales)、假单胞菌目(Pseudomonadales)、根瘤菌目(Rhizobiales)、芽孢杆菌目(Bacillales)、伯克氏菌目(Burkholderiales)、放线菌目(Actinomycetales)、海洋螺菌目(Oceanospirillales)、黄杆菌目(Flavobacteriales)、交替单胞菌目(Alteromonadales), 21个属。【结论】新疆阿克苏地区土壤中的微生物丰富度非常高, 存在大量的细菌类群, 优势菌群不尽相同, 盐碱土样中微生物群落结构与环境因子是密切相关的。

Abstract:

[Objective] The research was conducted to study on the bacterial community diversity and dominant population in Xinjiang akesu area and the correlation with the environmental factor. [Methods] The bacteria diversity and dominant population was analysis by PCR-denaturing gradient gel electrophoresis (DGGE), cloning, sequencing and the DGGE sequence analysis and the canonical correlation analysis (CCA). [Results] The sequencing of the dominant population showed that there were 29 sequencing belong to the un-cultivation microbial, and the other 43 DGGE band were made up by 9 order: Myxococcales, Pseudomonadales, Rhizobiales, Bacillales, Burkholderiales, Actinomycetales, Oceanospirillales, Flavobacteriale, Alteromonadales. [Conclusion] Canonical correlation analysis (CCA) result showed that microbial community structure and environmental factors were closely related in saline-alkaline soils.