肽核酸探针在微生物诊断领域的应用进展

Application of peptide nucleic acid probes in microbiological diagnostication

摘要:

肽核酸(Polyamide nucleic acid, PNA)是以中性酰胺键为骨架的脱氧核糖核酸(DNA)结构类似物, 它可以特异性地与DNA杂交, 且具有极高的生物稳定性。相比较传统的DNA探针技术, 肽核酸探针以其特殊的结构和性质在食品、环境及临床等微生物快速诊断领域显示出独特的优势。就肽核酸探针在微生物诊断方面的应用进展做简要综述。

Abstract:

Polyamide nucleic acid (PNA) molecules are DNA mimics with neutral acrylamide bonds as tskeleton. PNA could combine specifically with targeting DNA and the PNA-DNA duplex exhibits higher thermal stability than the corresponding DNA-DNA duplex. Due to their specific structure and characteristics, PNA probes have recently been developed and applied to a variety of microbiological diagnostication fields, such as food, environmental and clinical assay, which go above and beyond the possibility of DNA probes. Here we summarize the recent application of PNA probes in the realm of microbiological diagnostication.