CLSI M38-A2肉汤稀释法对蝙蝠蛾拟青霉(Paecilomyces hepiali)的药敏检测

Antifungal susceptibility test of Paecilomyces hepiali by CLSI M38-A2 broth dilution method

摘要:

【目的】对保健食品生产菌株蝙蝠蛾拟青霉(Paecilomyces hepiali)进行6种常见抗真菌药物: 伊曲康唑、酮康唑、伏立康唑、环吡酮胺、5-氟胞嘧啶、氟康唑的药敏检测。【方法】参考美国国家临床实验室标准化委员会CLSI M38-A2《肉汤稀释法抗真菌药敏试验参考方案》。【结果】质控菌株MIC值均在参考范围之内, 蝙蝠蛾拟青霉(P. hepiali)对以上6种药物的MIC值分别为1 mg/L、0.5 mg/L、0.25 mg/L、≥128 mg/L、64 mg/L、2 mg/L。【结论】M38-A2方案适合用来测定蝙蝠蛾拟青霉(P. hepiali)对抗真菌药物的敏感性, 但获得菌株耐药敏感性的判别标准, 仍需进一步的试验数据。

Abstract:

[Objective] To test filamentous fungi antifungal susceptibility, this study determined the minimal inhibitory concentration (MIC) values of 6 antifungal agents: Itraconazole (ITC), Ketoconazole (KET), Voriconazole (VRC), Ciclopirox (CIC), 5-Flurocytosine (5FC), Fluconazole (FLU) to the strain Paecilomyces hepiali which usually used in health food industry. [Methods] Method of Clinical Laboratory Standards Institute (CLSI) M38-A2 ＜Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi; Approved Standard—Second Edition＞ was referred. [Results] The results showed that the quality control strain Candida parapsilosis ATCC 22019T MIC values were within the reference range, the strain P. hepiali MIC values for the 6 antifungal agents were 1 mg/L, 0.5 mg/L, 0.25 mg/L, ≥128 mg/L, 64 mg/L, 2 mg/L respectively. [Conclusion] M38-A2 method could be applied to determine the susceptibility of P. hepiali to antifungal agents. But, more tests needed to acquire the standard interpretation of the strains susceptibility.