Abstract:[Objective] The aim of the present study was to identify the genera and screen potential characteristics bacteria in the lactic acid bacteria isolated from koumiss. [Methods] The characteristics of lactic acid bacteria were studied by the experiments on some aspects, such as morphology, physiological and biochemical characteristics, molecular biology and the inhibition of pathogenic bacteria. [Results] There were two strains of Lactobacillus plantarum, two strains of Enterococcus villorum, two strains of Enterococcus dispar, three strains of Enterococcus durans and one strain of Enterococcus raffinosus. The tested bacteria had different degrees of inhibition to Staphylococcus aureus, Escherichia coli and Enteritidis bacillus. [Conclusion] HZ24 and HZ25 were good potential probiotics which may make great contributions to food fermentation industry.