低pH处理对两歧双歧杆菌KLDS2.0603黏附能力的影响

Influence of low pH on the adhesion ability of Bifidobacterium bifidum KLDS2.0603

摘要:

【目的】确定低pH处理对两歧双歧杆菌KLDS2.0603黏附能力及其表面物理化学性质的影响。【方法】将两歧双歧杆菌KLDS2.0603菌体在不同低pH的PBS溶液中处理一定时间后, 采用平板菌落计数法和直接镜检法, 测定其经历不同pH的酸性环境后的黏附能力, 及其表面疏水性和自动聚集能力。【结果】不同pH的PBS溶液处理后的双歧杆菌菌体, 其黏附能力均不同程度下降, 除pH 5.0的处理组外, 其余处理组均显著低于空白组。此外, 经不同pH的PBS溶液处理后, 仅pH 3.0和3.5的两处理组, 双歧杆菌表面疏水性显著提高。除pH 1.0、1.5和5.0的处理组外, 其余处理组的自动聚集能力均显著下降。【结论】低pH的酸性环境会降低两歧双歧杆菌KLDS2.0603的黏附能力, 并且双歧杆菌的自动聚集能力和表面疏水性也发生相应变化。除pH 3.0和3.5的处理组外, 三者之间呈现一定的正相关性。

Abstract:

[Objective] The influence of low pH on the adhesion ability and the surface properties of Bifidobacterium bifidum KLDS2.0603 were determined in this paper. [Methods] After Bifidobacterium bifidum KLDS2.0603 was treated by PBS of different low pH, the adhesion ability was assayed by gram staining microscopic examination and plate colony counting method. The autoaggregation ability and surface hydrophobicity of Bifidobacterium bifidum KLDS2.0603 were also assayed. [Results] The adhesion abilities of Bifidobacterium bifidum KLDS2.0603 treated by PBS with different low pH were decreased. The adhesion abilities of treatment groups were significantly lower than control group except the group of pH 5.0. The surface hydrophobicity of Bifidobacterium bifidum KLDS2.0603 treated by PBS of pH 3.0 and 3.5 was significantly increased. Except for experimental groups of pH 1.0, 1.5 and 5.0, the autoaggregation ability of Bifidobacterium bifidum KLDS2.0603 was significantly reduced. [Conclusion] The adhesion ability of Bifidobacterium bifidum KLDS2.0603 was reduced in the low pH environment. The autoaggregation ability and surface hydrophobicity of Bifidobacterium bifidum KLDS2.0603 were also affected. The adhesion ability was positive correlation with the autoaggregation ability and surface hydrophobicity excluding the groups of the pH 3.0 and 3.5 for Bifidobacterium bifidum KLDS2.0603.