Isolation and character of radio-resistant black yeast-like fungus
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摘要:
【目的】确定辐射污染土样中分离筛选获得的一批黑色酵母状真菌的分类学地位和抗逆特性。【方法】通过菌落形态和菌丝特征观察, 结合LSU rDNA D1/D2区序列的系统发育分析, 并进行了菌株的60Co γ射线照射、紫外线照射和对等重金属离子以及NaCl生长压力实验。【结果】上述菌株均为Aureobasidium 属菌, 其中菌株F99和F134均与A. pullulans var. subglaciale CBS 123388T具有最大同源性, 为100%; 菌株F19 与A. pullulans var. melaogenum CBS 105.22T具有最大同源性, 为99.8%; 上述菌株均具有极强的抗逆特性。【结论】为了解耐辐射真菌抗逆机理提供了重要的研究材料。
Abstract:
[Objective] To identify the black yeast-like fungus isolated from the radiation-polluted soil and investigate the resistance. [Methods] the colony morphology and mycelium of isolates was observed, and phylogenetic tree was constructed based on LSU rDNA D1/D2 domain sequences. And then, the isolates resistance to 60Co gamma rays and ultraviolet rays and stress resistance to heavy metals and salt were analyzed. [Results] The results showed that isolates were varieties of Aureobasidium pullulans. The isolates F99 and F134 had a high similarity (100%) with A. pullulans var. subglaciale CBS 123388T as the closest relative, and the isolate F19 had a high similarity (100%) with A. pullulans var. melaogenum CBS 105.22T as the closest relative. Furthermore, isolates had higher radiation resistance and stress resistance. [Conclusion] This paper provides important materials to investigate the mechanism of radiation-resistant fungus.
