新型大肠杆菌高效表达载体pHsh的构建与应用

The approach to high production of recombinant protein via pHsh vectors for Escherichia coli

摘要:

在现代生物学和生物技术研究中, 通过基因的重组表达获得目标蛋白是一种应用最广泛的方法。因其培养简单、操作方便、遗传背景清楚、克隆表达系统成熟完善, 大肠杆菌表达系统通常是人们表达重组蛋白的首选, 而表达载体在重组蛋白的生产中起决定作用。pHsh及其衍生质粒是近年发展起来的新型大肠杆菌表达载体, 其调控外源基因表达的原理不同于所有其他表达系统, 并且具有表达水平高、成本低廉等特点。介绍大肠杆菌表达系统的组成和常用表达载体, 并对由pHsh系列载体组成的Hsh表达体系的构建策略、表达调控机制及其使用方法进行综述。Hsh表达体系的建立和发展有望从一个不同的角度帮助解决基因的重组表达中常见的表达水平低、诱导剂成本高、包涵体形成等问题。

Abstract:

For the production of recombinant proteins, Escherichia coli is often the first choice because it is easy, fast and inexpensive to cultivate, and its vector systems have been well developed. The regulation system for heterogenic expression plays an important role in producing recombinant proteins. pHsh vectors have been developed by using a heat-shock promoter of E. coli, and give advantages in high expression levels, low costs for expression induction, and reducing the formation of inclusion bodies. This paper introduces the construction strategy, expression mechanism, application of the pHsh vectors.