Abstract:[Objective] In order to study the spatial distribution of ammonia-oxidizing bacteria (AOB) in different vegetation communities (Tamarix ramosissima, Halocnemum strobilaceum, Phragmites australis) and different soil layers (0?5cm, 5 cm?15 cm, 15 cm?25 cm and 25 cm?35 cm) of wetland in the Ebinur Lake in Xinjiang. Then explore the correlations between the spatial distribution of AOB and soil environment factors. [Methods] MPN-Griess was used to study the distribution of AOB, using Pearson’s correlation analysis relationships between the distribution of AOB and soil environment factors. [Results] The distribution of AOB had obvious differences in vegetation communities of wetland in Ebinur Lake. The trend of distribution is that the highest Tamarix ramosissima community area, Halocnemum strobilaceum community is the second smallest and Phragmites australis community area minimum. The distribution of AOB also had obvious differences in different soil layers, the trend of distribution is that 15 cm?25 cm>0?5 cm>5 cm?15 cm>25 cm?35 cm. The distribution of AOB was significantly correlated to soil organic matter content, it had no correlation with pH, Moisture content, Salinity and NH4+?N content. [Conclusion] The distribution of AOB had obvious differences in vegetation communities and different soil layers of wetland in Ebinur Lake. Except the distribution of AOB showed obvious relationship with soil organic matter content, it had no relation with the other soil factors.