According to the college, students’ learning situation and the characteristics of curriculum, this paper discusses the influence of research-oriented teaching mode for food microbiology teaching. In the process of teaching reform, it emphasizes the students’ innovation ability and comprehensive quality training. It pays attention to the coordination of the theory teaching, experimental teaching and scientific research activities. At the same time it strengthens the process management. Teaching practice shows that the research-oriented teaching mode changes students learning way, and improve the scientific research ability and teaching quality obviously.