Abstract:Constructivism learning theory is increasingly recognized in higher education reform. Using this theory, we designed a new microbiological experiment teaching course including core and optional experi-ments, established a student-teacher interactive platform integrating the multimedia and network technologies, and propounded an evaluation system which is more comprehensive, objective and reli-able. The pilot demonstrated that this new paradigm can significantly arouse the participant interest and initiative, enhance the teaching efficiency and performance as well.