结合缺陷型菌株显色法采用离子束诱变筛选高产L-精氨酸突变株

Mutated by iron implantation for screening high L-arginine production strains using a plate coloration method by auxotroph strain

摘要:

为快速高效筛选L-精氨酸高产突变株, 建立一种缺陷菌株平板显色法并采用低能N+离子束对L-精氨酸生产用菌株钝齿棒杆菌SYPA5-5进行诱变处理, 通过上述平板显色法筛选获得高产突变株。对突变株进行摇瓶发酵实验, 最终选育出一株L-精氨酸产量较高且产酸性能比较稳定的突变菌株钝齿棒杆菌SYPA5-5-36。该菌株摇瓶发酵L-精氨酸产量可达35.85 g/L, 比出发菌株提高了19.5%。因此, 缺陷型菌株平板显色法可以用于快速、高效筛选高产L-精氨酸突变株。

Abstract:

A plate coloration method by auxotroph strain for screening high L-arginine production mutants quickly and efficiently was established. Coynebacterum crenatum SYPA5-5 which is an L-arginine producer was mutated by N+ ion implantation with an energy level of 10 keV. Combined with the directed screening method and fermentation in flasks, a mutant C. crenatum SYPA5-5-36 with a higher L-arginine production and stability was selected. The yield of L-arginine produced by this mutant could reach 35.85 g/L which was 19.5% higher than that of the original strain. Therefore, the plate coloration method by auxotroph strain could be applied to screening high L-arginine production mutants quickly and efficiently.