石油污染盐碱土壤翅碱蓬根围的细菌多样性及耐盐石油烃降解菌筛选

Bacterial diversity and halotolerant petroleum-degrading bacteria of the rhizosphere of Suaeda salsa (L.) in petroleum-contaminated saline-alkali soil

摘要:

为了更好地了解石油污染盐碱土壤翅碱蓬根围的细菌多样性, 采用16S rRNA基因克隆文库方法对其进行分析, 在此基础上采用富集培养方法从该生境中分离筛选耐盐石油烃降解菌。16S rRNA基因克隆文库分析结果表明, 海杆菌属(Marinobacter)、食烷菌属(Alcanivorax)和假单胞菌属(Pseudomonas)是该生境中的优势菌。他们可能在石油污染盐碱土壤翅碱蓬植物修复过程中起重要作用。进一步采用富集培养方法, 从该生境中分离得到8株耐盐石油烃降解菌, 可以耐受6%?10%浓度的NaCl, 石油烃降解率在32.3%?57.0%之间。经16S rRNA基因序列分析, 8株菌隶属于戈登氏菌属(Gordonia)、无色杆菌属(Achromobacter)、迪茨菌属(Dietzia)、芽胞杆菌属(Bacillus)和假单胞菌属(Pseudomonas)。他们可能参与石油污染盐碱土壤翅碱蓬植物修复过程中的石油烃降解。

Abstract:

Bacterial community associated with Suaeda salsa (L.) rhizosphere in petroleum-contaminated saline-alkali soil was investigated by 16S rRNA gene clone library analysis. Halotolerant petroleum-degrading bacteria were further isolated by enrichment using petroleum as the sole source of carbon and energy. Marinobacter, Alcanivorax and Pseudomonas dominated Suaeda salsa (L.) rhizosphere in petroleum-contaminated saline-alkali soil as revealed by 16S rRNA gene clone library analysis, which were likely to have a role in the phytoremediation of such soil by Suaeda salsa (L.). Eight halotolerant petroleum-degrading bacterial strains were isolated, which could grow up to 6%?10% NaCl, and could degrade 32.3% ?57.0% petroleum hydrocarbon in 3% NaCl over a period of 14 days. They were identified to belong to the genus of Gordonia, Achromobacter, Dietzia, Bacillus and Pseudomonas by 16S rRNA gene sequence analysis. They may involve in the degradation of petroleum in the phytoremediation of such soil by Suaeda salsa (L.).