In this paper, the teaching reform in the Biotechnology course presented in the Bioengineering Department of the Inner Mongolia University of Technology was discussed. In order to stimulate students’ interest in learning, train students’ abilities in studying and develop their team spirit, “Course design” and “Semi-self-help teaching model” were introduced into the Biotechnology course. In practice teaching section, “Specialty experiment” and “Comprehensive training” were employed to strengthen students’ abilities in operation and engineering practice. Through the practice of a few years, the teaching of Biotechnology has been improved constantly. The teaching reform has reached a good teaching effect.