紫色非硫细菌固体石蜡双层平板培养法

The paraffin wax overlay plate method for purple nonsulfur bacteria

摘要:

紫色非硫细菌是一类不产氧光合细菌, 具有重要的生态学意义和应用价值。建立固体石蜡双层平板法, 与传统的培养法相比较, 发现该方法可以更方便、更快捷地计数和分离紫色非硫细菌。结合分子生物学技术, 将该方法应用于水稻土紫色非硫细菌的研究, 发现以甲酸为碳源的固体石蜡双层平板法可以很好地对环境中可培养的紫色非硫细菌多样性进行研究。该方法的建立将更有助于紫色非硫细菌的研究。

Abstract:

Purple nonsulfur bacteria are one branch of purple phototrophic bacteria, which are ecologically important and have valuable applications. In this study, we established paraffin wax overlay plate method for the isolation and the enumeration of purple nonsulfur bacteria. Compared with several tra-ditional methods, the new one was found to be more rapid and convenient. We applied this method to-gether with molecular approaches to investigate purple nonsulfur bacteria in paddy soil, and found that it can well reveal the diversity of purple nonsulfur bacteria at culturable level, when formate acts as carbon source. We believe the new method would be of great help to the investigation of purple non-sulfur bacteria in environment.