苏云金芽孢杆菌双组份信号转导系统的生物信息学分析

Bioinformatics analysis of two-component signal transduction systems of Bacillus thuringiensis

摘要:

苏云金芽孢杆菌(Bacillus thuringiensis)能产生杀虫晶体蛋白等多种活性成分, 是目前应用最广泛的微生物杀虫剂。本文采用生物信息学方法, 系统分析了由本实验室完成全基因组测序的苏云金芽孢杆菌YBT-1520、CT-43和BMB171 3个菌株的双组分信号转导系统(Two-component signal transduction system, TCS)的分布、结构及功能, 并初步构建了部分TCS的调控网络关系图。本研究旨在为深入研究苏云金芽孢杆菌的生长、代谢以及毒力因子的表达与调控, 全面了解伴孢晶体的形成机制开辟新的研究方向。

Abstract:

Bacillus thuringiensis (Bt) strains, which can produce insecticidal crystal proteins, were widely used as biological pesticides. In this paper, we comprehensively analyzed the distributions, structures and putative biological functions of two-component transduction systems (TCS) from the genomes of Bt strains YBT-1520, CT-43 and BMB171, which have been sequenced by our laboratory. And more importantly, we constructed a preliminary TCS regulatory networks. This study should open a novel research direction in Bt for the growth, metabolism, regulator of toxic gene expression, as well as the formation mechanism of parasporal crystals.