CP1菌株的分离、筛选及其对毒死蜱的降解

Isolation, identification and degradative properties of CP1 for chlorpyrifos

摘要:

从生产毒死蜱的农药生产厂曝气池中分离、筛选到降解毒死蜱且能以毒死蜱为唯一碳源生长的微生物菌株, 命名为CP1。根据该菌株的Biolog特性鉴定和16S rRNA序列相似性分析, 初步鉴定该菌为苍白杆菌属(Ochrobactrum sp.)。利用正交实验和Box-Behnken响应面法对影响CP1菌株降解毒死蜱的主要因素进行优化分析, 得到菌株CP1对毒死蜱的最适降解条件为: 农药浓度100 mg/L, pH值7.0, 温度为28.5 °C。优化后, CP1对毒死蜱的降解率由最初的70.26%提高到75.18%。毒死蜱降解优化试验提高了CP1菌株对毒死蜱的生物降解性能。

Abstract:

A bacterium, named CP1, capable of effectively degrading chlorpyrifos was isolated from activated sludge from a pesticide plant. Based on the phylogenetic analysis of 16S rRNA gene and Biolog test, the isolate CP1 was preliminarily identified as a strain of Genus Ochrobactrum. Both the orthogonal experimental design and Box-Behnken design were employed to optimized the main factors which influences the chlorpyrifos-degrading efficiency by strain CP1. The optimum conditions for chlorpyrifos biodegradation were as follows: the initial concentration of chlorpyrifos was 100 mg/L, the pH was 7.0, and the culturing temperature was 28.5 °C. Under the optimum condition, the biodegrada-tion efficiency of chlorpyrifos increased from 70.26% to 75.18%. Therefore, the optimization of chlor-pyrifos-degrading condition could improve the biodegradation efficiency of chlorpyrifos by Ochrobac-trum sp. strain CP1.