恩诺沙星对4种水产致病弧菌的抑杀菌效应

Pharmacodynamics effect of enrofloxacin on four aquatic pathogenic vibrio

摘要:

采用二倍稀释法测定恩诺沙星对溶藻弧菌、最小弧菌、哈维氏弧菌、创伤弧菌4种12株菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC), 结果表明: 恩诺沙星对12株弧菌的MIC为0.1?0.8 mg/L, MBC为0.4?3.2 mg/L。在此基础上, 从每种菌中选取一株对恩诺沙星较为敏感的菌株, 研究恩诺沙星不同药物浓度(1 MIC、2 MIC、4 MIC)对4种弧菌的杀菌动力学和抗菌后效应(PAE), 结果显示: 各浓度恩诺沙星对溶藻弧菌X040625-R菌株均有较强的杀菌作用, 而对哈维氏弧菌M071202-H、创伤弧菌Q050723-C、最小弧菌H010911-Z等3菌株却表现出了低浓度抑菌、高浓度缓慢杀菌的作用。恩诺沙星的抗菌后效应PAE与药物浓度及细菌与药物的接触时间成正相关, 恩诺沙星对溶藻弧菌X040625-R的PAE最长, 对哈维氏弧菌M071202-H的PAE最短。

Abstract:

The minimal inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of enrofloxacin against 12 strains of Vibrio alginolyticus, V. mimicus, V. harveyi, V. vulnificus were determined by two-fold broth macrodilution method and the results showed that the MIC and MBC were 0.1?0.8 mg/L and 0.4?3.2 mg/L respectively. On the basis of these, 4 sensitive strains of 12 vibrio srtains were measured with the killing-curves and the PAE in different concentrations of enrofloxacin. The results showed that every concentration of enrofloxacin against V. alginolyticus X040625-R had a strong antibactericidal effect, but that against V. harveyi M071202-H, V. vulnificus Q050723-C, V. mimicus H010911-Z showed that the three bacteria was inhibited in the lower concentration and gradually killed with the increasing concentration. The PAE of enrofloxacin had a direct relationship with the concentration and the mixing time of the drug. The PAE of enrofloxacin against V. alginolyticus X040625-R was the longest while that against V. harveyi M071202-H was the shortest.