Problem-based learning is a problem-based and student-centered instructional model which takes professional ability as goal in teaching process. We have introduced it into the teaching of Food Microbiology in higher vocational school. As a result, it is evident that PBL does have a large impact on improving students’ motivation and subjectivity. It is also good for enhancing teachers’ leading station during teaching. At the same time, many other abilities have been greatly improved with the application of PBL.