娄彻氏链霉菌ATCC10739产抗生素Borrelidin发酵条件优化及其分离纯化

Borrelidin production by Streptomycete rochei ATCC10739: optimization of fermentation, separation and purification conditions

摘要:

对娄彻氏链霉菌ATCC10739固体发酵产生十八元大环内酯抗生素Borrelidin进行了发酵条件的优化。首先筛选得到了理想的发酵培养基; 其次考察了发酵时间、起始pH值以及在ISP-2培养基中添加附加碳源、氮源对Borrelidin产量的影响。初步确定最适发酵条件为: ISP-2培养基中添加1%甘油, 起始pH值为6.0, 培养温度30 °C, 发酵时间为7 d, 产量可达1.336 mg/L。采用有机溶剂萃取、硅胶层析和半制备型高效液相色谱(HPLC)等分离技术纯化得到Borrelidin。

Abstract:

The solid fermentation conditions, including fermentation medium, fermentation time, initial pH, as well as additional carbon and nitrogen source for borrelidin produced by Streptomycete rochei ATCC10739 were investigated. Under the optimal fermentation conditions (ISP-2 medium supplemented with 1% glycerol; initial pH 6.0; culture temperature 30 °C; fermentation time, 7 days), the highest borrelidin production level was achieved as 1.336 mg/L media. Borrelidin was separated and purified by organic extraction, followed by silica gel chromatography and semipreparative high performance liquid chromatography (HPLC).