两种光合细菌生物转化槲寄生培养液菌体中几种同工酶的变化

Changes of several isoenzymes of biomass in two kinds of photosynthetic bacteria biotransformation mistletoe culture fluid

摘要:

采用聚丙烯酰胺凝胶电泳法对两种光合细菌的生物转化槲寄生培养液中菌体的蛋白质和几种同工酶进行研究, 并以纯光合细菌培养液中菌体作对照。结果表明, 光合细菌生物转化槲寄生过程中, 两种光合细菌的蛋白质、酯酶同工酶和过氧化物酶同工酶均发生改变, 某些蛋白质、酯酶和过氧化物酶的合成受到抑制, 并有新的蛋白质、酯酶和过氧化物酶生成; 超氧化物歧化酶的表达未明显改变。由此可见, 槲寄生能诱导光合细菌合成新的酯酶和过氧化物酶, 这些诱导酶可能参与了槲寄生的生物转化。本研究为光合细菌生物转化槲寄生转化机理的研究及槲寄生在抗肿瘤领域的进一步应用奠定了基础。

Abstract:

Polyacrylamide gel electrophoresis was used for studying the protein and several isoenzymes of biomass in two kinds of photosynthetic bacteria (PSB) transforming mistletoe culture fluid (PSBT), pure PSB culture fluid as control. The proteinogram and the zymograms demonstrated that proteins, esterase isoenzyme (EST) and peroxidase isoenzyme (POD) of PSB in PSBT changed, i.e. the expression of some proteins, EST and POD of PSB were inhibited, while some new proteins, EST and POD of PSB in PSBT appeared. The expression of superoxide dismutase (SOD) of PSB in PSBT did not alter evidently. The results showed that PSB could be induced to generate new protiens, EST and POD by mistletoe, and which might take part in biotransformation of mistletoe. The research provided basis for studying the biotransformation mechanism of mistletoe by PSB and the further application of mistletoe to tumor therapy.