萝卜对土生空团菌菌丝生长的影响

Influence of Raphanus sativus L. on the mycelium growth of Cenococcum geophilum Fr.

摘要:

纯培养条件下, 测定了十字花科植物萝卜(Raphanus sativus L.)种子、幼苗、根系分泌物及幼苗提取物对土生空团菌(Cenococcum geophilum Fr. (Cg))菌株CgSO1、CgSB2、CgO5、SPOP2 和Cg5#菌株生长的影响。结果表明供试Cg菌株与萝卜种子共培养, 或将萝卜根系分泌物和幼苗提取物加入到培养基中, 均促进了Cg菌丝生长。高温灭菌处理使萝卜根系分泌物和幼苗提取物对Cg菌株的促生作用更强, 而高温灭菌后的萝卜幼苗段对菌株生长影响不大。其中经高温灭菌处理的幼苗水提取物对5菌株的促生作用最大, CgSO1、CgSB2、CgO5、SPOP2和Cg5#每菌落的菌丝干重分别达到: 54.8、45.8、63.9、41.2和50.5 mg。

Abstract:

The influence of radish (Raphanus sativus L.) seeds, seedlings, root exudates, and seedling extracts on the mycelium growth of Cenococcum geophilum Fr. (Cg) was investigated in vitro. The mycelium growth of Cg strains (CgSO1, CgSB2, CgO5, SPOP2 and Cg5#) was promoted, when Cg strains were co-cultured with radish seedlings, or cultured with root exudates, and water extract of radish seedlings. However, Cg mycelium growth was significantly improved by the autoclaved (treated under high temperature) root exudates or autoclaved water extract of radish seedlings, while the autoclaved seedlings segments did not. Results showed that the maximum mycelium dry biomass of the 5 strains of Cg was given in the medium added the autoclaved water extract of the seedlings. The single colony dry maximum biomass of each Cg strain (CgSO1, CgSB2, CgO5, SPOP2 and Cg5#) was reached to 54.8, 45.8, 63.9, 41.2 and 50.5 mg respectively.