基于宏微观结合的甾体微生物羟化反应中菌体形态的表征

Characterization of fungal morphology based on the combination of macroscopic and microscopic view in steroid microbial hydroxylation

摘要:

为考察生长调节剂对甾体微生物转化过程中菌体生长状况的影响, 利用沉降密度宏观定量表征菌体的形态和扫描电镜微观观测菌体形态相结合的方法, 描述了生长过程中菌体形态的变化。结果表明: 与未加调节剂的空白体系对照, 加调节剂体系菌体在各个生长时期均呈现出较小的沉降密度。通过SEM图像可以明显看出加调节剂体系菌体表面光滑, 长势丰满、挺拔, 生长状况优良。两种方法均表明调节剂的加入明显促进了菌体的生长。

Abstract:

To research the effects of growth regulator on the status of mycelia growth during steroid biotransformation, morphology of filamentous microorganisms was characterized in combination with both sedimentary density method in macroscopic view, and using Scanning Electron Microscope (SEM) method in microscopic view during the whole strain growth phases. The results showed that in comparison with strain growing without adding growth regulator, the strains growing, which was added growth regulator, showed relatively lower sedimentation densities during all different stages than the one not added. Through SEM, it can be clearly seen that the bacteria with the regulator presents better conditions such as smooth surfaces, rounded plump as well as excellent shapes. Both methods prove adding regulator are able to promote the exuberant growth of the bacteria.